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News Feature pollution caused E. coli disease outbreak – water pollution, E. coli – the environmental protection industry

Incidence 2008 10 23, Boluo Town, Guangdong Province, one of the 6,000 chicken farmers keep three yellow chicken, 5 days old inoculated a new combined vaccine support, 7 days a H5 influenza vaccine inoculation , 10 days old seedlings water bursal immune disease began 12 days of age, of death, the greater physical die sooner. Local veterinary diagnosis of E. coli disease, and tylosin treatment with doxycycline did not receive good results, then according to drug susceptibility test: rifampin, metronidazole, neomycin, etc. still no clear advantage of high efficacy, by 9 days keep medication every day mortality 20 to 30 chickens, chicken body getting weaker, market owners and veterinarians are very anxious. Coincided with author visits in Huizhou.

Clinical symptoms Chickens spirit gloomy, poor appetite, loose feathers disorder and emaciation. Some of the chicken neck stretched mouth breathing, there are rales, conjunctival inflammation, corneal opacity, blindness, loose stool, behind a white or yellow green loose bowel movements, and feet, staggering, walking swing, paw skin shrinkage. Some coma to death, and some fell to the ground before his death, overturned the abdomen, legs, back done, beat their wings, and finally death struggle.

Necropsy thickening dead or sick birds can be seen airbags, muddy and not clear and turbid with sticky exudates or caseous exudate. Pericardial thickening, opacity, adhesion, pericardial and epicardial there is a white fibrous material attached. Pericardium and abdominal cavity filled with yellow fluid. Enlargement of the liver surface with a layer of yellow-white fibrin attachment. Intestinal catarrhal inflammation, mucosal bleeding, ulcers, intestine a large number of mucus and gas.

Laboratory Smear sterile dead chickens to diseases of liver, spleen, blood smear, Gram stain, microscopic examination shows that a large number of Gram-negative, medium size, rounded at both ends, the whole body of the short flagellum bacterium. Isolation of bacteria disease

dead chickens taken the hard work and spleen were inoculated blood agar plate agar slant and Maikang Kai, the culture was observed after 24 hours. Grown on blood agar in gray, moist, slightly elevated, edge of neat, do not micro uplift hemolytic colonies; in Maikang Kai agar formed round, smooth, uplift, central depression of the red colony. Get colony smear, staining for the short Gram-negative bacteria.

Treatment According to the patients, clinical symptoms, pathological changes in laboratory E. coli disease can be diagnosed. However, the exact cure for the disease, detailed observations of the farm’s environment, identify the pathogen source. The staff reflects the chance that the chicken is always the last few days with diarrhea, I went to the living and the chicken shared water tower and found a thick layer of bottom water tower biofilm, showing that this tower over from lack of clean-up, leading to water contamination, resulting in large intestine bacillus disease outbreaks, the more water the chicken disease more serious.

Take measures to (1) audience without water, disinfecting sheds, thoroughly cleaned, disinfected water towers, clear the surface of the biofilm.

(2) drug use: re-use of clean water against the drug to chickens, selected Prudential strong Hercules (amikacin) 100 ml of dilute water of 100 liters, 500 ml chicken with Victory Duishui 1500 l for 5 days; toppings: 5% glucose and 0.05% vitamin C, 5 days.

Results

Treated, the disease has been effectively controlled, the next day reduced the mortality rate of chickens more than half the number of deaths to eight, mental status improved; by 5 days after treatment, flock mental state returned to normal, basically stopped dead , feeding, and drinking water has fully achieved the level of normal chickens. However, the disease lasted ten days, more than 300 chickens died only to the farmers caused great economic losses. Recommendation: for safety reasons, a day added to drinking water in the chicken (2 ~ 5) × 10E-6 of peracetic acid to kill E. coli, and regularly clean disinfected water tower.

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